منابع مشابه
Protein anisotropy turns solubility on its head.
I n the effort to understand living cells, knowledge about molecular details competes with knowledge of collective properties of large systems of molecules. On the one hand, molecular structures and interactions strongly suggest how the cell’s molecular machines work and give rise to rich metabolic diagrams. On the other hand, subtle collective properties such as the entropy and free energy rep...
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For many years, the aggregation of proteins and polypeptides remained a neglected area of protein chemistry. It was only with the discovery that the insoluble deposits found in the organs and tissues of patients suffering from different diseases were enriched in a single, but different polypeptide, that the interest in understanding how and why these proteins aggregate arose (Fernàndez-Busquets...
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1. Introduction A major impediment to the production of recombinant proteins in Escheri-chia coli is their tendency to accumulate in the form of insoluble and biologically inactive aggregates known as inclusion bodies. Although it is sometimes possible to convert aggregated material into native, biologically-active protein , this is a time consuming, labor-intensive, costly, and uncertain under...
متن کاملSequence-based prediction of protein solubility.
In order to investigate the relationship between the thermodynamics and kinetics of protein aggregation, we compared the solubility of proteins with their aggregation rates. We found a significant correlation between these two quantities by considering a database of protein solubility values measured using an in vitro reconstituted translation system containing about 70% of Escherichia coli pro...
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ژورنال
عنوان ژورنال: Nature Biotechnology
سال: 2001
ISSN: 1087-0156,1546-1696
DOI: 10.1038/84325